New to single-cell? We are here to get you started smoothly on your first single-steps.
Single-cell can unveil new critical biological information that is not to be missed on. Enhance your study and use single-cell to:
New to single-cell? We are here to get you started smoothly on your first single-steps.
Single-cell can unveil new critical biological information that is not to be missed on. Enhance your study and use single-cell to:
![New to singlecell Bienfait 1 Heterogeneity](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/icons/New_to_singlecell_Bienfait_1_Heterogeneity_2024-02-21-132943_yfdv.png)
Uncover tissue heterogeneity
Obtain an accurate representation of your cell populations and identify new cell subtypes in healthy tissues
![New to singlecell Bienfait 2 New pathways](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/icons/New_to_singlecell_Bienfait_2_New_pathways_2024-02-21-132948_zypr.png)
Describe new pathways
Characterize gene regulatory networks and cell-cell interactions contributing to tissue-specific mechanisms and functions
![New to singlecell Bienfait 3 Disease](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/icons/New_to_singlecell_Bienfait_3_Disease_2024-02-21-132954_cghp.png)
Understand diseases better
Identify the regulation of inflammation, measure the abnormal representation of cell subtypes and profile infiltrating cells
![New to singlecell Bienfait 4 Target](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/icons/New_to_singlecell_Bienfait_4_Target_2024-02-21-132959_qkkr.png)
Identify therapeutic targets
Pinpoint promising signaling pathways and cell transition states for the development of new treatments
![New to singlecell Bienfait 5 Diagnostics](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/icons/New_to_singlecell_Bienfait_5_Diagnostics_2024-02-21-133006_hvxo.png)
Improve diagnostics
Identify cell gene signatures and biomarkers for resistance to existing therapies for custom care
![New to singlecell Bienfait 6 3 D culture](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/icons/New_to_singlecell_Bienfait_6_3D_culture.png)
Validate 3D culture models
Obtain accurate reproduction of cell populations compared to a real sample and verify the similarity of transcriptomic profiles
![New to Single Cell Is singlecell for me](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/_AUTOxAUTO_crop_center-center_none/12525/New_to_Single_Cell_Is_singlecell_for_me.webp)
Is single-cell for me?
If your samples possibly contains cellular diversity and you already have experience with molecular biology, or if you already work with bulk RNA-seq, then single-cell is waiting for you!
You will only need:
- Samples with at least 10,000 cells, preferably fresh for authentic biological representation,
- A tissue dissociation protocol to obtain whole cells
- Access to a sequencing platform
Take a look at the key steps below!
![New Pre Steps 2x](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/New-PreSteps%402x_2024-02-15-160259_cjrr.png)
Dissociate your biopsy or cell culture sample into a highly viable single-cell suspension.
1
![New Step1 2x](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/New-Step1%402x.png)
Using a single-cell sample preparation method, like Asteria, to isolate and barcode the transcriptome of your individual cells.
2
![New Step2 2x](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/New-Step2%402x.png)
Perform standard reverse-transcription on the captured mRNA and PCR amplification to generate high amounts of barcoded cDNA.
3
![New Step3 2x](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/New-Step3%402x.png)
Create your Illumina librairies and sequence your barcoded cDNA at your local core platform to obtain FASTQ files.
4
![New Step4 2x](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/New-Step4%402x.png)
Analyze your FASTQ files with the Cytonaut software to generate differential gene expression analysis, to sort your cell populations in clusters, and more.
![New After Steps 2x](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/New-AfterSteps%402x_2024-02-15-160336_pbac.png)
Explore your transcriptomic data, and use your Cytonaut publication-ready figures to share your new biological discoveries!
![Astronaute reflexion square 450x400](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/astronaute_reflexion_square_450x400.png)
Still hesitating?
Our aim is two-fold:
- provide a technology to ensure the original, authentic transcriptome is captured – no cell fixation, no freezing, no vortexing like other kits!
- make single-cell accessible, with a hassle-free setup for every labWith a straightforward benchtop kit for sample preparation and an intuitive analysis software, it has never been so easy to extract and explore critical single-cell data!
![Astronaute TEESHIRT v2 Square 450x400](https://aws-scipio-web-s3-craft.s3.eu-west-3.amazonaws.com/_AUTOxAUTO_crop_center-center_none/15735/astronaute_TEESHIRT_v2_Square_450x400.webp)
A risk-free trial kit is waiting for you
To help you get started, we have a special offer on your first Asteria kit, including access to the Cytonaut software. Also, as you succeed with your first single-cell project, you would be gifted a custom T-shirt with your own data on it!
In the meantime, we also have put together a “think before you start” cheat sheet in our resource center. Do not forget to also check the FAQ!