Asteria™

Benchtop 3’ scRNA-seq

Sample Preparation Kit

Cell wellness by design with our high-performance hydrogel-based technology

Asteria Hero Image
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Asteria Bienfait 1 Pictogram Fresh

Fresh transcriptome

Protect the original transcriptome by always  processing fresh cells, removing the need for fixation, vortexing or freezing steps

Asteria Bienfait 2 Pictogram Benchtop

Benchtop-based

Process cells as you go, instrument-free, and use only what you need! Convenient for work with Infectious samples

Asteria Bienfait 3 Pictogram Simple

Simple Protocol

Perform stress-free, training-free and risk-free sample preparation

Asteria Bienfait 4 Pictogram Autonomy

Complete autonomy

Obtain your amplified cDNA rapidly in 14 hours, with only 7 hours of hands-on time for multiple samples

Asteria Bienfait 5 Pictogram Stopping Point

Flexible stopping points

Control your schedule by stabilizing your samples early and resuming your protocol on another day and/or another location

Asteria Bienfait 6 Pictogram Fragile

Safe for fragile cells

Cushion your cells in a hydrogel and avoid stressful mechanics – preserve your cell populations!

Asteria Bienfait 1 Pictogram Fresh

Fresh transcriptome

Protect the original transcriptome by always  processing fresh cells, removing the need for fixation, vortexing or freezing steps

Asteria Bienfait 2 Pictogram Benchtop

Benchtop-based

Process cells as you go, instrument-free, and use only what you need! Convenient for work with Infectious samples

Asteria Bienfait 3 Pictogram Simple

Simple Protocol

Perform stress-free, training-free and risk-free sample preparation

Asteria Bienfait 4 Pictogram Autonomy

Complete autonomy

Obtain your amplified cDNA rapidly in 14 hours, with only 7 hours of hands-on time for multiple samples

Asteria Bienfait 5 Pictogram Stopping Point

Flexible stopping points

Control your schedule by stabilizing your samples early and resuming your protocol on another day and/or another location

Asteria Bienfait 6 Pictogram Fragile

Safe for fragile cells

Cushion your cells in a hydrogel and avoid stressful mechanics – preserve your cell populations!

Asteria Section Echantillons Ultimate Transcriptome

Offering you the ultimate authentic transcriptome

We remove the historical contradiction between kit-based flexibility and the need to fix, vortex or freeze cells, all of which can have dramatic consequences damaging your transcriptomic data, altering gene expression, or even removing entire cell populations.

Our hydrogel-based technology RevGel protects your cells, enabling access to a transcriptome representative of the biological conditions.

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A simple workflow

Asteria Pre Steps 2x

Once your single-cell suspension is ready, transfer it into the tube with barcoded beads

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Asteria Step1 2x
Gently mix your cells with our barcoded beads to form 1:1 pairs before dilution in the liquid hydrogel

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Asteria Step2 2x
Polymerize the hydrogel by simply placing the tube on ice, creating a gentle physical mesh isolating the cell-bead pairs

3

Asteria Step3 2x
Lyse your cells, capturing cytoplasmic mRNA transcripts onto the local barcoded bead

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Asteria Step4 2x
Dissolve the hydrogel for easy collection of the beads carrying the transcriptomic information, then proceed with classic reverse transcription and cDNA amplification
Asteria After Steps 2x

Prepare your libraries and sequence your single-cell data at your sequencing facility

Asteria easy workflow C Orrected Axis

An easy workflow for high performance

Decipher the heterogeneity of your cell populations confidently with Asteria’s ~45% capture rate (on a benchmark mix of HEK293/NIH3T3), with over 12K transcripts and over 5K genes per human cell.

Asteria has proven powerful across many sample types. Check out some single-cell projects below!

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Asteria Seamless Collaboration

Seamless collaboration with your core platform

Thanks to Asteria’s flexible workflow, the sample can be handed over to the core platform for sequencing at different timepoints. Hand over the lysed cells in the hydrogel, or perform the molecular biology steps yourself to generate and amplify the cDNA!

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Product Cytonaut Block

Cytonaut™

An intuitive data analysis tool for everyone

Discover Cytonaut™, Asteria’s companion, providing you with the entire single-cell data analysis pipeline to process your raw sequencing data all the way to figures ready for publication.

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