Here you will find the report on our internal study finding no differences by freezing a sample at the lysis step and storing it at -80°C for 7 or 35 days compared to immediate processing. This provides great flexibility in planning and scheduling your experiments, enabling the processing of fresh samples upon arrival no matter the timing.
With this early stopping point in the workflow, you can start processing a fresh sample immediately to preserve the fresh transcriptome then safely pause two hours later. You can resume the experiment the next day, the following week, or even the following month without fear of affecting your transcriptomic data.
It also gives the flexibility to start processing and storing samples as they arrive, then thaw them together and perform the molecular biology steps simultaneously to save time at the bench and reduce a possible batch effect.