Single-cell skin samples prepared with Asteria™ and analysed with Cytonaut™ win ESDR 2022 Poster Prize
On the 1st of October 2022, Raya Poncin (PhD, R&D Project Leader at Scipio bioscience) and the Skin Immunity and Inflammation team at the Institut de Recherche Saint-Louis (IRSL) presented at the prestigious 51st European Society for Dermatological Research (ESDR) meeting in Amsterdam. The ESDR hosts an annual conference gathering world leaders and pioneers in skin sciences and dermatology, where a selected number of posters are presented live. This year, out of 725 submissions, 126 were chosen for live presentation, and 20 won the ESDR Poster Prize, one being ESDR279, of which conclusive results on skin cell heterogeneity and complexity were obtained using the Asteria™ benchtop kit for sample preparation and Cytonaut™ cloud software for data analysis.
The single-cell skin samples poster titled “Instrument-free single-cell resolution of transcriptome in human skin” is the result of a collaboration with the ISRL at the Saint Louis Hospital in Paris, with notable participation from Sarah D, Chloé G, Marine M, David B, Jean-David B, and Hélène LB.
From healthy skin samples (epidermis and dermis), single-cell RNA suspensions were prepared using the RevGel-seq™ technology, the main protocol powering Scipio bioscience’s Asteria™ kit. Following standard single-cell workflows (reverse transcription, PCR amplification, and cDNA sequencing), count matrix generation, cell clustering and 2D embedding were automatically performed by Cytonaut™. Clusters were then manually annotated thanks to the interactive data visualization module of Cytonaut, Cytonaut Rover, by exploring the gene expression of published biomarkers. These manual annotations were then validated against automated annotation independently performed using the SingleR method supervised by a reference dataset.
Thus, feasibility of instrument-free cell sample preparation with Asteria™ and downstream analysis with Cytonaut™ was demonstrated, allowing clear observations on the heterogeneity and the complexity of human skin samples. In follow-up experiments, more important sequencing depth and work on pathological cell samples could enable higher diversity in cell subtype characterization.
Article authored by Yann Zhong from Scipio bioscience.