Currently, the Asteria™ single-cell RNAseq kit has been validated to process mammalian cells, including primary and cultured cell lines.
Yes, the Asteria™ single-cell RNA seq kit features a stopping point 2 hours after starting the protocol. The sample can then be safely stored at -80 °C for three days without loss of transcriptomic information. Stability for a longer period is being tested and further data will be made available soon.
The use of Asteria™ single-cell RNA seq kit only requires basic cell and molecular biology laboratory equipment. A shortlist is available below,
please refer to the User Guide for more details.
• PCR thermocycler
• Centrifuge
• Cell counting equipment (microscope and C-chips from Nanotek are recommended)
• Orbital shaker
• Benchtop centrifuge for 1.5 mL microtubes
• Mini-centrifuge for 1.5 mL microtubes
• Rotator/wheel for 1.5 mL microtubes
• Calibrated Micropipettes (P10, P20, P200, P1000)
• Vortex mixer
• Sterile and nuclease-free micropipette filtered tips (low-retention tips are recommended)
• DNase/RNase-free 1.5 mL microtubes
• DNase/RNase-free PCR strips
• Molecular biology grade DNase/RNase-free water
• 0.1M NaOH solution (freshly prepared from Molecular biology grade stock solution)
We invite you to find the contact of your local distributor on this page. If no distributor yet covers your country, we will deliver the kit to you ourselves and we invite you to fill out our contact form here.